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FokI - 版本历史
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<p><b>新页面</b></p><div>{{Infobox protein family<br />
| Symbol = Endonuc-Fok1_C<br />
| Name = Restriction endonuclease Fok1, C terminal<br />
| image = 1fok.png<br />
| width = <br />
| caption = Fok1结合DNA的[[Protein Data Bank|PDB]]图像：{{PDBe|1fok}}<ref>{{Cite journal <br />
| last1 = Aggarwal | first1 = A. K. <br />
| last2 = Wah | first2 = D. A. <br />
| last3 = Hirsch | first3 = J. A. <br />
| last4 = Dorner | first4 = L. F. <br />
| last5 = Schildkraut | first5 = I. <br />
| title = Structure of the multimodular endonuclease Fok1 bound to DNA <br />
| url = https://archive.org/details/sim_nature-uk_1997-07-03_388_6637/page/96 | journal = Nature <br />
| volume = 388 <br />
| issue = 6637 <br />
| pages = 97–100 <br />
| year = 1997 <br />
| doi = 10.1038/40446 <br />
| pmid = 9214510 <br />
| pmc = <br />
}}</ref><br />
| Pfam = PF09254<br />
| Pfam_clan = CL0415<br />
| InterPro = IPR015334<br />
| SMART = <br />
| PROSITE = <br />
| MEROPS =<br />
| SCOP = 2fok <br />
| TCDB = <br />
| OPM family = <br />
| OPM protein = <br />
| CAZy = <br />
| CDD =<br />
}}<br />
'''FokI'''是一種存在於細菌''Flavobacterium okeanokoites''的type IIS限制酶，含有位於[[N端]]的[[DNA結合區塊]]（N-terminal DNA-binding domain），以及一個位於[[C端]]的非專一性DNA切割區塊<ref name="Durai 2005">{{cite journal en | author = Durai S, Mani M, Kandavelou K, Wu J, Porteus M, Chandrasegaran S | title = Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells | journal = Nucleic Acids Res | volume = 33 | issue = 18 | pages = 5978-90 | year = 2005 | id = PMID 16251401 | url = http://nar.oxfordjournals.org/cgi/content/full/33/18/5978 | access-date = 2007-07-20 | archive-date = 2007-05-10 | archive-url = https://web.archive.org/web/20070510101730/http://nar.oxfordjournals.org/cgi/content/full/33/18/5978 | dead-url = no }}</ref>。當此酵素與[[DNA]]辨識並結合於5'-GGATG-3'[[识别位点]]時，將會把位於結合位置下游的9到13個[[核苷酸]]切除（不需特定序列）<ref name="Wah 1998">{{cite journal en| last=Wah| first= D. A.| coauthors=J. Bitinaite, Schildkraut, I., Aggarwal, A. K.| year=1998| title=Structure of FokI has implications for DNA cleavage| journal=Proc Natl Acad Sci U S A| volume=95| issue=18| pages=10564-9}}</ref>。''FokI''的分子量為65.4 kDA，含有587個[[氨基酸]]。<br />
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== 參考文獻 ==<br />
{{reflist|2}}<br />
{{Restriction enzyme}}<br />
<br />
[[Category:限制酶]]</div>
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