https://arolstar52-zhtest.hf.space/index.php?action=history&feed=atom&title=EcoRI
EcoRI - 版本历史
2026-07-17T17:27:18Z
在这个wiki上该页的修订历史
MediaWiki 1.43.9
https://arolstar52-zhtest.hf.space/index.php?title=EcoRI&diff=321390&oldid=prev
imported>Isoleemina：将”酵素”修改为”酶”，并添加超链接，由于酵素这一说法现已几乎不用，因此作此改动
2025-11-27T08:53:25Z
<p>将”酵素”修改为”酶”，并添加超链接，由于酵素这一说法现已几乎不用，因此作此改动</p>
<p><b>新页面</b></p><div>[[File:EcoRI restriction enzyme recognition site.svg|thumb|220px|''Eco''RI切位。]][[File:ECOR1_Crystal_Structure.rsh.png|thumb|220px|EcoRI分子晶體結構圖，左側是橙色與綠色部分是DNA鏈。]]<br />
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'''''Eco''RI'''（「I」是羅馬數字「1」）是一種[[核酸酶]]，來自某些特定品系的[[大腸桿菌]]（''E. coli''，也是其名稱由來），是此種細菌體內參與[[限制修飾系統]]的[[酶]]。<br />
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在[[分子生物學]]或其他分子層次的生物學研究上，EcoRI是一種常用的[[限制酶]]，可將特定序列的[[DNA]]切割成片段，切割後的小片段端點為5'端突出的[[黏狀末端]]。<br />
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== 參見 ==<br />
*[[EcoRV]]<br />
*[[FokI]]<br />
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== 參考文獻 ==<br />
*Bitinaite, J., D. A. Wah, Aggarwal, A. K., Schildkraut, I. (1998). "FokI dimerization is required for DNA cleavage." Proc Natl Acad Sci U S A 95(18): 10570-5.<br />
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*Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.<br />
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*McClarin, J.A., Frederick, C.A., Wang, B.C., Greene, P., Boyer, H.W., Grable, J., Rosenberg, J.M. (1986) Structure of the DNA-EcoRI endonucleases recognition complex at 3 Å resolution, Science. 234. 4783, 1526-1542.<br />
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*Kurpiewski, M.R., Engler, L.E., Wozniak, L.A., Kobylanska, A., Koziolkiewicz, M., Stec, W.J, Jen-Jacobson, L. (2004) Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases, Structure 12. 1775-1788.<br />
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== 外部連結 ==<br />
* [http://biologicalworld.com/digestions.htm Restriction Enzyme Digestion of Plasmid DNA]{{Wayback|url=http://biologicalworld.com/digestions.htm |date=20071221012557 }}<br />
* {{MeshName|Eco-RI}}<br />
{{Restriction enzyme}}<br />
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[[Category:EC 3.1.21]]<br />
[[Category:分子生物學]]<br />
[[Category:细菌酶]]<br />
[[Category:限制酶]]</div>
imported>Isoleemina