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{{Infobox_gene}}

'''CCAAT/增强子结合蛋白β'''，简称'''C/EBP-β'''或'''CEBPB'''，化学式为：C1603H2495O481N449S11<ref>{{Cite web |title=Expasy - ProtParam |url=https://web.expasy.org/cgi-bin/protparam/protparam_bis.cgi?P17676@1-345@ |website=web.expasy.org |access-date=2026-01-24}}</ref>，是一种在人体中由''CEBPB''[[基因]]编码的[[蛋白质]]。<ref name="pmid1535333">{{cite journal |vauthors=Szpirer C, Riviere M, Cortese R, Nakamura T, Islam MQ, Levan G, Szpirer J |date=Jul 1992 |title=Chromosomal localization in man and rat of the genes encoding the liver-enriched transcription factors C/EBP, DBP, and HNF1/LFB-1 (CEBP, DBP, and transcription factor 1, TCF1, respectively) and of the hepatocyte growth factor/scatter factor gene (HGF) |journal=Genomics |volume=13 |issue=2 |pages=293–300 |doi=10.1016/0888-7543(92)90245-N |pmid=1535333}}</ref><ref name="pmid1840554">{{cite journal |vauthors=Cao Z, Umek RM, McKnight SL |date=Oct 1991 |title=Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells |journal=Genes Dev |volume=5 |issue=9 |pages=1538–52 |doi=10.1101/gad.5.9.1538 |pmid=1840554 |doi-access=free}}</ref>

== 功能 ==
这种无[[内含子]]基因编码的蛋白质是一种[[BZIP结构域|bZIP]]转录因子，可以作为同源[[二聚体]]与某些[[DNA]]调控区域结合。它还可以与相关蛋白 [[CEBPA|C/EBP-α]]、[[CEBPD|C/EBP-δ]]和[[CEBPG|C/EBP-γ]]形成异二聚体。编码的蛋白质在调节涉及免疫和炎症反应的基因中很重要，并且已显示与[[白细胞介素-6|IL-6]]基因中的[[白细胞介素1族|IL-1]]反应元件以及几种急性期和[[细胞因子]]基因的调节区域结合。此外，编码的蛋白质可以结合[[启动子]]和上游元件并刺激[[I型胶原蛋白]]基因的表达。<ref>{{cite web |title=Entrez Gene: CEBPB CCAAT/enhancer binding protein (C/EBP), beta |url=https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1051 }}</ref>

CEBPB对正常的[[巨噬细胞]]功能至关重要，巨噬细胞是一种重要的[[免疫系统|免疫]]细胞[[白血球|亚型]]；无法[[基因表達|表达]]CEBPB的小鼠具有无法[[细胞分化|分化]]（特化）的巨噬细胞，因此无法执行其所有生物学功能——包括巨噬细胞介导的肌肉修复。<ref name="ruffel_mice">{{cite journal |vauthors=Ruffell D, Mourkioti F, Gambardella A, Kirstetter P, Lopez RG, Rosenthal N, Nerlov C |year=2009 |title=A CREB-C/EBPbeta cascade induces M2 macrophage-specific gene expression and promotes muscle injury repair |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=106 |issue=41 |pages=17475–80 |bibcode=2009PNAS..10617475R |doi=10.1073/pnas.0908641106 |pmc=2762675 |pmid=19805133 |doi-access=free}}</ref>[[流行病学|观察]]工作表明，血液白细胞中CEBPB的表达与人体肌肉力量呈正相关，<ref name="harries_cebpb">{{cite journal |vauthors=Harries LW, Pilling LC, Hernandez LD, Bradley-Smith R, Henley W, Singleton AB, Guralnik JM, Bandinelli S, Ferrucci L, Melzer D |year=2012 |title=CCAAT-enhancer-binding protein-beta expression in vivo is associated with muscle strength |journal=Aging Cell |volume=11 |issue=2 |pages=262–8 |doi=10.1111/j.1474-9726.2011.00782.x |pmc=3486692 |pmid=22152057}}</ref>强调了免疫系统，特别是巨噬细胞在维持肌肉功能中的重要性。

CEBPB基因的功能可以通过基于独立验证的[[siRNA]]敲低来有效检查。<ref>{{Cite journal |last1=Munkácsy |first1=Gyöngyi |last2=Sztupinszki |first2=Zsófia |last3=Herman |first3=Péter |last4=Bán |first4=Bence |last5=Pénzváltó |first5=Zsófia |last6=Szarvas |first6=Nóra |last7=Győrffy |first7=Balázs |date=2016-01-01 |title=Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments |journal=Molecular Therapy: Nucleic Acids |language=en |volume=5 |issue=9 |pages=e366 |doi=10.1038/mtna.2016.66 |issn=2162-2531 |pmc=5056990 |pmid=27673562}}</ref>

== 靶基因 ==
CEBPB能够增加几个靶基因的表达。其中，一些在[[神经系统]]中具有特定作用，如[[TAC1|前速激肽原-1]]基因，产生[[P物质]]和[[神经激肽A]]<ref name="pmid16771829">{{cite journal |vauthors=Kovács KA, Steinmann M, Magistretti PJ, Halfon O, Cardinaux JR |date=September 2006 |title=C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones |journal=J. Neurochem. |volume=98 |issue=5 |pages=1390–9 |doi=10.1111/j.1471-4159.2006.03957.x |pmid=16771829 |s2cid=36225447}}</ref>以及负责生物合成重要[[神经递质]][[乙酰胆碱]]的[[胆碱乙酰转移酶]]。<ref name="pmid12393272">{{cite journal |vauthors=Robert I, Sutter A, Quirin-Stricker C |date=October 2002 |title=Synergistic activation of the human choline acetyltransferase gene by c-Myb and C/EBPbeta |journal=Brain Res. Mol. Brain Res. |volume=106 |issue=1–2 |pages=124–35 |doi=10.1016/S0169-328X(02)00419-9 |pmid=12393272}}</ref>其他目标包括编码细胞因子的基因，例如[[白细胞介素-6|IL-6]]、<ref name="pmid1730090">{{cite journal |vauthors=Natsuka S, Akira S, Nishio Y, Hashimoto S, Sugita T, Isshiki H, Kishimoto T |date=January 1992 |title=Macrophage differentiation-specific expression of NF-IL6, a transcription factor for interleukin-6 |url=https://archive.org/details/sim_blood_1992-01-15_79_2/page/460 |journal=Blood |volume=79 |issue=2 |pages=460–6 |doi=10.1182/blood.V79.2.460.460 |pmid=1730090 |doi-access=free}}</ref>[[白细胞介素-4|IL-4]]、<ref name="pmid7594540">{{cite journal |vauthors=Davydov IV, Krammer PH, Li-Weber M |date=December 1995 |title=Nuclear factor-IL6 activates the human IL-4 promoter in T cells |url=https://archive.org/details/sim_journal-of-immunology_1995-12-01_155_11/page/5272 |journal=J. Immunol. |volume=155 |issue=11 |pages=5273–9 |pmid=7594540}}</ref>[[白细胞介素-5|IL-5]]、<ref name="pmid10453008">{{cite journal |vauthors=van Dijk TB, Baltus B, Raaijmakers JA, Lammers JW, Koenderman L, de Groot RP |date=September 1999 |title=A composite C/EBP binding site is essential for the activity of the promoter of the IL-3/IL-5/granulocyte-macrophage colony-stimulating factor receptor beta c gene |url=https://archive.org/details/sim_journal-of-immunology_1999-09-01_163_5/page/2674 |journal=J. Immunol. |volume=163 |issue=5 |pages=2674–80 |pmid=10453008}}</ref>和[[肿瘤坏死因子-α]]。<ref name="pmid10629048">{{cite journal |vauthors=Greenwel P, Tanaka S, Penkov D, Zhang W, Olive M, Moll J, Vinson C, Di Liberto M, Ramirez F |date=February 2000 |title=Tumor Necrosis Factor Alpha Inhibits Type I Collagen Synthesis through Repressive CCAAT/Enhancer-Binding Proteins |url=https://archive.org/details/sim_molecular-and-cellular-biology_2000-02_20_3/page/912 |journal=Mol. Cell. Biol. |volume=20 |issue=3 |pages=912–8 |doi=10.1128/MCB.20.3.912-918.2000 |pmc=85208 |pmid=10629048}}</ref>还发现编码赋予细胞多药抗性的转运蛋白的基因被CEBPB激活。这样的基因包括[[多药耐药相关蛋白2|ABCC2]]<ref name="pmid10573531">{{cite journal |vauthors=Tanaka T, Uchiumi T, Hinoshita E, Inokuchi A, Toh S, Wada M, Takano H, Kohno K, Kuwano M |date=December 1999 |title=The human multidrug resistance protein 2 gene: functional characterization of the 5'-flanking region and expression in hepatic cells |url=https://archive.org/details/sim_hepatology_1999-12_30_6/page/1507 |journal=Hepatology |volume=30 |issue=6 |pages=1507–12 |doi=10.1002/hep.510300617 |pmid=10573531 |s2cid=22514353}}</ref>和[[P-糖蛋白|ABCB1]]。<ref name="pmid15044620">{{cite journal |vauthors=Chen GK, Sale S, Tan T, Ermoian RP, Sikic BI |date=April 2004 |title=CCAAT/enhancer-binding protein beta (nuclear factor for interleukin 6) transactivates the human MDR1 gene by interaction with an inverted CCAAT box in human cancer cells |journal=Mol. Pharmacol. |volume=65 |issue=4 |pages=906–16 |doi=10.1124/mol.65.4.906 |pmid=15044620 |s2cid=86591291}}</ref>

== 相互作用 ==
CEBPB已被证明可以与以下物质相互作用：

* [[CREB1]]<ref name="pmid12773552">{{cite journal |vauthors=Chen Y, Zhuang S, Cassenaer S, Casteel DE, Gudi T, Boss GR, Pilz RB |year=2003 |title=Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires cooperation between CREB and C/EBP-beta |url=https://archive.org/details/sim_molecular-and-cellular-biology_2003-06_23_12/page/4066 |journal=Mol. Cell. Biol. |volume=23 |issue=12 |pages=4066–82 |doi=10.1128/mcb.23.12.4066-4082.2003 |pmc=156132 |pmid=12773552}}</ref>
* [[CRSP3]]<ref name="pmid14759369">{{cite journal |vauthors=Mo X, Kowenz-Leutz E, Xu H, Leutz A |date=January 2004 |title=Ras induces mediator complex exchange on C/EBP beta |journal=Mol. Cell |volume=13 |issue=2 |pages=241–50 |doi=10.1016/s1097-2765(03)00521-5 |pmid=14759369 |doi-access=free}}</ref>
* [[DDIT3]]<ref name="pmid12706815">{{cite journal |vauthors=Hattori T, Ohoka N, Hayashi H, Onozaki K |date=April 2003 |title=C/EBP homologous protein (CHOP) up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform |journal=FEBS Lett. |volume=541 |issue=1–3 |pages=33–9 |doi=10.1016/s0014-5793(03)00283-7 |pmid=12706815 |s2cid=43792576 |doi-access=free}}</ref><ref name="pmid8662954">{{cite journal |vauthors=Fawcett TW, Eastman HB, Martindale JL, Holbrook NJ |date=June 1996 |title=Physical and functional association between GADD153 and CCAAT/enhancer-binding protein beta during cellular stress |url=https://archive.org/details/sim_journal-of-biological-chemistry_1996-06-14_271_24/page/14284 |journal=J. Biol. Chem. |volume=271 |issue=24 |pages=14285–9 |doi=10.1074/jbc.271.24.14285 |pmid=8662954 |doi-access=free}}</ref>
* [[EP300]]<ref name="pmid9343424">{{cite journal |vauthors=Mink S, Haenig B, Klempnauer KH |date=November 1997 |title=Interaction and functional collaboration of p300 and C/EBPbeta |url=https://archive.org/details/sim_molecular-and-cellular-biology_1997-11_17_11/page/6609 |journal=Mol. Cell. Biol. |volume=17 |issue=11 |pages=6609–17 |doi=10.1128/mcb.17.11.6609 |pmc=232514 |pmid=9343424}}</ref>
* [[雌激素受体α]]<ref name="pmid9817600" /><ref name="pmid7651415">{{cite journal |vauthors=Stein B, Yang MX |date=September 1995 |title=Repression of the interleukin-6 promoter by estrogen receptor is mediated by NF-kappa B and C/EBP beta |url=https://archive.org/details/sim_molecular-and-cellular-biology_1995-09_15_9/page/4971 |journal=Mol. Cell. Biol. |volume=15 |issue=9 |pages=4971–9 |doi=10.1128/mcb.15.9.4971 |pmc=230744 |pmid=7651415}}</ref>
* [[糖皮质激素受体]]<ref name="pmid9817600">{{cite journal |vauthors=Boruk M, Savory JG, Haché RJ |date=November 1998 |title=AF-2-dependent potentiation of CCAAT enhancer binding protein beta-mediated transcriptional activation by glucocorticoid receptor |journal=Mol. Endocrinol. |volume=12 |issue=11 |pages=1749–63 |doi=10.1210/mend.12.11.0191 |pmid=9817600 |doi-access=free}}</ref>
* [[HMGA1]]<ref name="pmid12665574" />
* [[热休克因子1|HSF1]]<ref name="pmid11801594">{{cite journal |vauthors=Xie Y, Chen C, Stevenson MA, Auron PE, Calderwood SK |date=April 2002 |title=Heat shock factor 1 represses transcription of the IL-1beta gene through physical interaction with the nuclear factor of interleukin 6 |url=https://archive.org/details/sim_journal-of-biological-chemistry_2002-04-05_277_14/page/11802 |journal=J. Biol. Chem. |volume=277 |issue=14 |pages=11802–10 |doi=10.1074/jbc.M109296200 |pmid=11801594 |doi-access=free}}</ref>
* [[核仁连续体磷蛋白1]]<ref name="pmid9553145">{{cite journal |vauthors=Miau LH, Chang CJ, Shen BJ, Tsai WH, Lee SC |date=April 1998 |title=Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation |url=https://archive.org/details/sim_journal-of-biological-chemistry_1998-04-24_273_17/page/10784 |journal=J. Biol. Chem. |volume=273 |issue=17 |pages=10784–91 |doi=10.1074/jbc.273.17.10784 |pmid=9553145 |doi-access=free}}</ref>
* [[RELA]]<ref name="pmid12707271">{{cite journal |vauthors=Weber M, Sydlik C, Quirling M, Nothdurfter C, Zwergal A, Heiss P, Bell S, Neumeier D, Ziegler-Heitbrock HW, Brand K |date=June 2003 |title=Transcriptional inhibition of interleukin-8 expression in tumor necrosis factor-tolerant cells: evidence for involvement of C/EBP beta |url=https://archive.org/details/sim_journal-of-biological-chemistry_2003-06-27_278_26/page/23586 |journal=J. Biol. Chem. |volume=278 |issue=26 |pages=23586–93 |doi=10.1074/jbc.M211646200 |pmid=12707271 |doi-access=free}}</ref><ref name="pmid9570146">{{cite journal |vauthors=Xia C, Cheshire JK, Patel H, Woo P |date=December 1997 |title=Cross-talk between transcription factors NF-kappa B and C/EBP in the transcriptional regulation of genes |url=https://archive.org/details/sim_international-journal-of-biochemistry-cell-biology_1997-12_29_12/page/1525 |journal=Int. J. Biochem. Cell Biol. |volume=29 |issue=12 |pages=1525–39 |doi=10.1016/s1357-2725(97)00083-6 |pmid=9570146}}</ref>
* [[血清反应因子]]<ref name="pmid10318842">{{cite journal |vauthors=Hanlon M, Sealy L |date=May 1999 |title=Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta |url=https://archive.org/details/sim_journal-of-biological-chemistry_1999-05-14_274_20/page/14224 |journal=J. Biol. Chem. |volume=274 |issue=20 |pages=14224–8 |doi=10.1074/jbc.274.20.14224 |pmid=10318842 |doi-access=free}}</ref><ref name="pmid9032301">{{cite journal |vauthors=Sealy L, Malone D, Pawlak M |date=March 1997 |title=Regulation of the cfos serum response element by C/EBPbeta |url=https://archive.org/details/sim_molecular-and-cellular-biology_1997-03_17_3/page/1744 |journal=Mol. Cell. Biol. |volume=17 |issue=3 |pages=1744–55 |doi=10.1128/mcb.17.3.1744 |pmc=231899 |pmid=9032301}}</ref>
* [[SMARCA2]]<ref name="pmid10619021">{{cite journal |vauthors=Kowenz-Leutz E, Leutz A |date=November 1999 |title=A C/EBP beta isoform recruits the SWI/SNF complex to activate myeloid genes |journal=Mol. Cell |volume=4 |issue=5 |pages=735–43 |doi=10.1016/s1097-2765(00)80384-6 |pmid=10619021 |doi-access=free}}</ref>
* [[Sp1 transcription factor]]<ref name="pmid12665574">{{cite journal |vauthors=Foti D, Iuliano R, Chiefari E, Brunetti A |year=2003 |title=A nucleoprotein complex containing Sp1, C/EBP beta, and HMGI-Y controls human insulin receptor gene transcription |url=https://archive.org/details/sim_molecular-and-cellular-biology_2003-04_23_8/page/2720 |journal=Mol. Cell. Biol. |volume=23 |issue=8 |pages=2720–32 |doi=10.1128/mcb.23.8.2720-2732.2003 |pmc=152545 |pmid=12665574}}</ref><ref name="pmid12847250">{{cite journal |vauthors=Liu YW, Tseng HP, Chen LC, Chen BK, Chang WC |date=July 2003 |title=Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of IL-10 in mouse macrophages |url=https://archive.org/details/sim_journal-of-immunology_2003-07-15_171_2/page/820 |journal=J. Immunol. |volume=171 |issue=2 |pages=821–8 |doi=10.4049/jimmunol.171.2.821 |pmid=12847250 |doi-access=free}}</ref>
* [[TRIM28]],<ref name="pmid11711437">{{cite journal |vauthors=Rooney JW, Calame KL |year=2001 |title=TIF1beta functions as a coactivator for C/EBPbeta and is required for induced differentiation in the myelomonocytic cell line U937 |url=https://archive.org/details/sim_genes-development_2001-11-15_15_22/page/3022 |journal=Genes Dev. |volume=15 |issue=22 |pages=3023–38 |doi=10.1101/gad.937201 |pmc=312827 |pmid=11711437}}</ref><ref name="pmid9742105">{{cite journal |vauthors=Chang CJ, Chen YL, Lee SC |date=October 1998 |title=Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression |url=https://archive.org/details/sim_molecular-and-cellular-biology_1998-10_18_10/page/5880 |journal=Mol. Cell. Biol. |volume=18 |issue=10 |pages=5880–7 |doi=10.1128/mcb.18.10.5880 |pmc=109174 |pmid=9742105}}</ref>
* [[EGR1]]<ref name="pmid12947119">{{cite journal |vauthors=Zhang F, Lin M, Abidi P, Thiel G, Liu J |date=November 2003 |title=Specific interaction of Egr1 and c/EBPbeta leads to the transcriptional activation of the human low density lipoprotein receptor gene |url=https://archive.org/details/sim_journal-of-biological-chemistry_2003-11-07_278_45/page/44246 |journal=J. Biol. Chem. |volume=278 |issue=45 |pages=44246–54 |doi=10.1074/jbc.M305564200 |pmid=12947119 |doi-access=free}}</ref>

== 参见 ==

* [[Ccaat-enhancer-binding proteins]]

== 参考文献 ==
{{reflist|colwidth=35em}}

== 外部链接 ==

* {{UCSC gene info|CEBPB}}
* {{MeshName|CEBPB+protein,+human}}
* {{FactorBook|CEBPB}}
{{转录因子与细胞内受体|g1}}

CEBPB - 版本历史

imported>曦城星月 来自 2026年1月24日 (六) 11:46

imported>曦城星月来自 2026年1月24日 (六) 11:46