编辑“︁EcoRI”︁

[[File:EcoRI restriction enzyme recognition site.svg|thumb|220px|''Eco''RI切位。]][[File:ECOR1_Crystal_Structure.rsh.png|thumb|220px|EcoRI分子晶體結構圖，左側是橙色與綠色部分是DNA鏈。]]

'''''Eco''RI'''（「I」是羅馬數字「1」）是一種[[核酸酶]]，來自某些特定品系的[[大腸桿菌]]（''E. coli''，也是其名稱由來），是此種細菌體內參與[[限制修飾系統]]的[[酶]]。

在[[分子生物學]]或其他分子層次的生物學研究上，EcoRI是一種常用的[[限制酶]]，可將特定序列的[[DNA]]切割成片段，切割後的小片段端點為5'端突出的[[黏狀末端]]。

== 參見 ==
*[[EcoRV]]
*[[FokI]]

== 參考文獻 ==
*Bitinaite, J., D. A. Wah, Aggarwal, A. K., Schildkraut, I. (1998). "FokI dimerization is required for DNA cleavage." Proc Natl Acad Sci U S A 95(18): 10570-5.

*Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.

*McClarin, J.A., Frederick, C.A., Wang, B.C., Greene, P., Boyer, H.W., Grable, J., Rosenberg, J.M. (1986) Structure of the DNA-EcoRI endonucleases recognition complex at 3 Å resolution, Science. 234. 4783, 1526-1542.

*Kurpiewski, M.R., Engler, L.E., Wozniak, L.A., Kobylanska, A., Koziolkiewicz, M., Stec, W.J, Jen-Jacobson, L. (2004) Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases, Structure 12. 1775-1788.

== 外部連結 ==
* [http://biologicalworld.com/digestions.htm Restriction Enzyme Digestion of Plasmid DNA]{{Wayback|url=http://biologicalworld.com/digestions.htm |date=20071221012557 }}
* {{MeshName|Eco-RI}}
{{Restriction enzyme}}

[[Category:EC 3.1.21]]
[[Category:分子生物學]]
[[Category:细菌酶]]
[[Category:限制酶]]