编辑“︁C2BBe1”︁

'''C2BBe1'''（亦稱'''ATCC CRL-2102'''、'''Caco-2BBe'''）是對[[表皮生長因子]]呈[[陽性反應]]的[[人類]]結腸腺癌[[細胞系]] ，最初是在1988年通過{{le|有限稀釋法|Dilution cloning}}，從一名72歲[[白人]][[男性]]結腸腺癌[[患者]]的[[Caco-2細胞]][[克隆]]出來而建系<ref name=a>{{cite journal |last1=Peterson |first1=MD |last2=Mooseker |first2=MS |title=Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. |journal=Journal of cell science |date=1992-07 |volume=102 ( Pt 3) |pages=581-600 |pmid=1506435 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/1506435/ |accessdate=2019-12-11}}</ref>。

==特徴==
C2BBe1細胞會形成[[極性]]且形態與人類[[結腸]]形態相似的單層[[細胞]]，故而早已應用於研究[[細菌]]的黏附和侵襲<ref>{{cite journal |last1=Huang |first1=Y |last2=Adams |first2=MC |title=An in vitro model for investigating intestinal adhesion of potential dairy propionibacteria probiotic strains using cell line C2BBe1. |journal=Letters in applied microbiology |date=2003 |volume=36 |issue=4 |pages=213-6 |doi=10.1046/j.1472-765x.2003.01303.x |pmid=12641713 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/12641713/ |accessdate=2019-12-11}}</ref><ref>{{cite journal |last1=Kops |first1=SK |last2=Lowe |first2=DK |last3=Bement |first3=WM |last4=West |first4=AB |title=Migration of Salmonella typhi through intestinal epithelial monolayers: an in vitro study. |journal=Microbiology and immunology |date=1996 |volume=40 |issue=11 |pages=799-811 |doi=10.1111/j.1348-0421.1996.tb01145.x |pmid=8985935 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/8985935/ |accessdate=2019-12-11}}</ref>。分離的頂端{{le|刷狀緣|Brush border}}其實包含着{{le|微絨毛蛋白|Villin}}、{{le|絲束蛋白|Fimbrin}}、{{le|蔗糖酶-異麥芽糖酶|Sucrase-isomaltase}}、 刷狀緣[[肌凝蛋白]]-1、{{le|胞襯蛋白|Fodrin}}及肌凝蛋白-2等[[物質]]<ref name=a/>。C2BBe1細胞表達的{{le|刷狀緣|Brush border}}[[肌凝蛋白]]-I的水平與人類[[大腸|腸]][[上皮組織|上皮細胞]]相似，並且比親本Caco-2細胞株更加均勻<ref name=a/>，但是C2BBe1細胞在[[微絨毛]]的[[長度]]及部分刷狀緣蛋白表達[[水平]]方面仍然是不相同的。C2BBe1細胞目前可與[[小鼠]][[派亞氏淋巴叢]]細胞共同培養，從而建立[[微皺褶細胞]][[物質模型|模型]]<ref>{{cite journal |last1=Corr |first1=S |last2=Hill |first2=C |last3=Gahan |first3=CG |title=An in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of Listeria monocytogenes across M cells. |journal=Microbial pathogenesis |date=2006-12 |volume=41 |issue=6 |pages=241-50 |doi=10.1016/j.micpath.2006.08.003 |pmid=17049432 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/17049432/ |accessdate=2019-12-11}}</ref>。

== 參考資料 == 
{{Reflist|2}} 

== 外部連結 == 
*[https://web.expasy.org/cellosaurus/CVCL_1096 Cellosaurus entry for C2BBe1]{{Wayback|url=https://web.expasy.org/cellosaurus/CVCL_1096 |date=20191211093533 }} 

[[Category:人類細胞系]]