编辑“︁DAPI”︁（章节）

==活细胞安全性==
===毒性===
DAPI可用于固定细胞染色，但是因为活细胞染色对DAPI浓度有严格要求，它很少被用于活细胞染色。<ref>{{cite journal |vauthors=Zink D, Sadoni N, Stelzer E |title=Visualizing Chromatin and Chromosomes in Living Cells. Usually for the live cells staining Hoechst Staining is used. DAPI gives a higher signal in the fixed cells compare to Hoechst Stain but in the live cells Hoechst Stain is used. |doi=10.1016/S1046-2023(02)00289-X|journal=Methods |volume=29 |issue=1 |pages=42–50 |year=2003 |PMID=12543070}}</ref>虽然DAPI能快速進入活細胞中與DNA結合<ref name="膜穿透性" />，但是[[材料安全性数据表|MSDS]]<ref>{{Cite web |url=http://www.kpl.com/docs/msds/710301.pdf |title=存档副本 |accessdate=2018-04-18 |archive-date=2016-03-03 |archive-url=https://web.archive.org/web/20160303211317/http://www.kpl.com/docs/msds/710301.pdf |dead-url=yes }}</ref>和其他文献<ref name="膜穿透性" />认为DAPI没有毒性。

有研究表明，大剂量的DAPI可以导致小鼠中毒死亡,LD<sub>50</sub>= 基质 37.3 mg/kg。<ref>{{cite journal|author=Slieger RF et al.|title=DAPI对鼠疟的治疗作用.pdf|doi=10.13389/j.cnki.rao.2013.11.001|url=http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD7984&filename=GWJC198104015&uid=WEEvREcwSlJHSldTTEYzVDhsN3d4K24xZ0JTUVh5cnNodGZxcGVBRWpKdz0=$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtwkF4VYPoHbKxJw!!&v=MTAxOTdiL0lJanJCYmJLeEZ0RE1xNDlFWVlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMT2ZZdWRwRkNqblU=|year=1980|access-date=2019-05-25|archive-date=2019-09-24|archive-url=https://web.archive.org/web/20190924221559/http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFD7984&filename=GWJC198104015&uid=WEEvREcwSlJHSldTTEYzVDhsN3d4K24xZ0JTUVh5cnNodGZxcGVBRWpKdz0=$9A4hF_YAuvQ5obgVAqNKPCYcEjKensW4IQMovwHtwkF4VYPoHbKxJw!!&v=MTAxOTdiL0lJanJCYmJLeEZ0RE1xNDlFWVlSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVJMT2ZZdWRwRkNqblU=|dead-url=no}}</ref>
===潜在的诱变性===
DAPI被視為一種[[致癌物]]。尽管DAPI没有显现出对[[大腸桿菌]]的诱变性<ref>{{cite journal |vauthors=Ohta T, Tokishita S, Yamagata H |title=Ethidium bromide and SYBR Green I enhance the genotoxicity of UV-irradiation and chemical mutagens in E. coli. |journal=Mutat. Res. |volume=492 |issue=1–2 |pages=91–7 |year=2001 |pmid=11377248 |doi=10.1016/S1383-5718(01)00155-3}}</ref>，在机器制造商提供的信息上，DAPI被认为是一种DNA诱变剂<ref name="DAPI Nucleic Acid Stain" />。由于DAPI可以掺入DNA螺旋中，它很可能具有底层的DNA诱变性，在使用過程中應注意配制、使用與拋棄的處理程序。